GE Healthcare provides transformational medical technologies and services that are shaping a new age of patient care. As part of the GE Healthcare BioProcess™ media platform, Capto ImpRes ion exchangers are manufactured under validated production methods, backed up with Regulatory Support Files, and have Secure Supply routines in place. "Capto ImpRes media have been designed to meet the throughput and high resolution needs of intermediate purification and polishing."Ĭapto ImpRes media are available in several convenient formats, including Predictor™ 96-well filter plates and prepacked HiTrap™ and HiScreen™ columns to support all stages of biopharmaceutical development and manufacture. "The extension of the Capto platform with Capto ImpRes ion exchangers demonstrates our commitment to supporting biopharmaceutical manufacturers with the most modern tools to improve their processes in terms of throughput, productivity, and economy," said Dr Nigel Darby, Vice President, Biotechnologies, GE Healthcare. The first Capto ImpRes products to be launched are Capto SP ImpRes and Capto Q ImpRes, strong cation and strong anion exchangers, respectively, that enable high throughput, high resolution polishing, and flexibility in process design.Ĭapto ImpRes media are based on a rigid high-flow agarose matrix that allows the use of high flow rates and higher bed heights, providing biopharmaceutical manufacturers with the possibility to reduce cycle times and maintain or reduce column equipment footprint, thus contributing to driving improvements in process economics. Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values.Uppsala, Sweden – GE Healthcare, a unit of General Electric Company (NYSE: GE), announced today that the capabilities of the well-established Capto platform are now extended to include Capto ImpRes ion exchange chromatography media for late-stage purification of a wide range of biomolecules. Wash with at least 2 CV ultra pure water.ĥ. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.Ĥ. Stable to commonly used aqueous buffers, 1.0 M NaOH 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.ġ. This prevents clogging and increases the life time of the column when loading large sample volumes. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.Ģ. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. These columns are easily connected in series to increase bed height to 20 cm.įast results and minimal consumption of sample and buffer consumption through the small bed volume.īioProcess columns packed with the same chromatography resin and using the same linear fluid velocity can produce scalable, reproducible results.ġ. Prepacked with Capto Q strong anion exchange chromatography resin.Ĭonvenient method optimization and parameter screening because of the 10cm bed height. HiScreen Capto Q is a column packed with a strong anion exchange modern resin, combining speed and capacity.
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